Biophysics lies at the interface among biology, physics and chemistry. The physical properties of biomolecules are responsible for the molecular characteristics of biological processes. Understanding how these biomolecules operate in life activities demands investigations through multidisciplinary approaches, including determinations of the atomic structures, characterizations of the kinetics and energy, constructions of the theories and models, etc. This course will offer an overview of the biomolecular structures and the physicochemical mechanisms for organizing these structures, then describe the methods for characterizing the structure and dynamics of biomolecules, and discuss the “structure-function” relationship in molecular biology.